Primer

Part:BBa_K2309032:Design

Designed by: Yijing Gong   Group: iGEM17_XJTLU-CHINA   (2017-09-27)


Standard reverse primer for Gibson Assembly to pNZ8148


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 22
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 22
    Illegal NotI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 22
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Rules for Gibson Assembly.


Source

No

References